Medium data
Search for medium no. = [544].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
544 THERMODESULFOVIBRIO HYDROGENIPHILUS MEDIUM
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NH4Cl | 1.0 | g |
| NaCl | 1.0 | g |
| Na2SO4 | 3.0 | g |
| KCl | 0.1 | g |
| CaCl2 | 0.1 | g |
| MgCl2·6H2O | 0.5 | g |
| Yeast extract | 0.1 | g |
| Sodium acetate | 0.16 | g |
| Trace element solution (see Medium No. 439) | 1.0 | ml |
| NaHCO3 | 2.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Na2S·9H2O | 0.4 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix ingredients except NaHCO3, L-cysteine·HCl·H2O and Na2S·9H2O and adjust pH to 7.2. Distribute the medium into tubes (e.g., 5 ml of the medium in the Hungate tubes) under a H2-CO2 gas mixture (4:1, v/v), seal with butyl rubber stoppers and autoclave. After cooling, add solutions of NaHCO3 [5%, filter-sterilized and stored under a N2-CO2 (4:1, v/v) gas mixture], L-cysteine·HCl·H2O and Na2S·9H2O (5%, autoclaved and stored under N2) to the medium. Pressurize the inoculated tubes to 200 kPa H2-CO2 (4:1, v/v).
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