Medium data

Search for medium no. = [544].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

544   THERMODESULFOVIBRIO HYDROGENIPHILUS MEDIUM

KH2PO4  0.3     g
K2HPO4  0.3     g
NH4Cl  1.0     g
NaCl  1.0     g
Na2SO4  3.0     g
KCl  0.1     g
CaCl2  0.1     g
MgCl2·6H2O  0.5     g
Yeast extract  0.1     g
Sodium acetate  0.16    g
Trace element solution (see Medium No. 439)  1.0     ml
NaHCO3  2.0     g
L-Cysteine·HCl·H2O  0.5     g
Na2S·9H2O  0.4     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix ingredients except NaHCO3, L-cysteine·HCl·H2O and Na2S·9H2O and adjust pH to 7.2. Distribute the medium into tubes (e.g., 5 ml of the medium in the Hungate tubes) under a H2-CO2 gas mixture (4:1, v/v), seal with butyl rubber stoppers and autoclave. After cooling, add solutions of NaHCO3 [5%, filter-sterilized and stored under a N2-CO2 (4:1, v/v) gas mixture], L-cysteine·HCl·H2O and Na2S·9H2O (5%, autoclaved and stored under N2) to the medium. Pressurize the inoculated tubes to 200 kPa H2-CO2 (4:1, v/v).