Medium data
Search for medium no. = [543].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
543 TINDALLIA TEXCOCONENSIS MEDIUM
Solution A:
| NH4Cl | 1.0 | g |
| KH2PO4 | 0.3 | g |
| KCl | 0.1 | g |
| CaCl2 | 0.1 | g |
| MgCl2·6H2O | 0.1 | g |
| NaCl | 75.0 | g |
| Yeast extract | 0.5 | g |
| Peptone | 5.0 | g |
| Resazurin | 1.0 | mg |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Distilled water | 700.0 | ml |
Mix components and adjust pH to 9.0 with 10 N KOH. Autoclave the solution anaerobically under a N2 atmosphere.
Solution B:
| NaHCO3 | 20.0 | g |
| Na2CO3 | 3.0 | g |
| Distilled water | 300.0 | ml |
Prepare the medium anaerobically under a N2 atmosphere.
To complete the medium, combine Solutions A and B under a N2 atmosphere. Prior to inoculation, add 0.5 g/L (final) L-cysteine·HCl·H2O and 0.4 g/L (final) Na2S·9H2O from anaerobic and sterile stock solutions (5.0%).
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