Medium data
Search for medium no. = [524].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
524 METHANE OXIDIZING BACTERIUM MEDIUM
| NaCl | 30.0 | g |
| KH2PO4 | 0.14 | g |
| CaCl2·2H2O | 0.8 | g |
| MgSO4·7H2O | 3.4 | g |
| MgCl2·6H2O | 4.2 | g |
| KCl | 0.33 | g |
| NaNO3 | 0.25 | g |
| NH4Cl | 0.25 | g |
| Na2SiO3·9H2O | 0.2 | g |
| Fe2(SO4)3·xH2O | 20.0 | mg |
| Na2SeO3·5H2O | 0.5 | mg |
| NiCl2·6H2O | 0.5 | mg |
| Trace mineral solution (see Medium No. 523) | 3.0 | ml |
| Distilled water | 1.0 | L |
Mix components, adjust pH to 6.0 - 6.5 and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium:
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
| 5% NaHCO3 solution | 10.0 | ml |
| 1 mM CuSO4 solution | 2.0 | ml |
Distribute the medium into culture vessels (leave >80% volume gas phase) under a N2-CO2 (4:1) gas mixture and seal with butyl rubber stoppers. Pressurize the culture vessels to 200 kPa with methane gas. Finally add O2 gas to make up 2% O2 concentration in the gas phase. Readjust pH to 6.0 - 6.3, if necessary.
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