Medium data

Search for medium no. = [519].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

519 SYNTROPHOMONAS MEDIUM (for Syntrophospora cellicola 19J-3)

Solution A:

Mineral solution (see below)	`50.0`	ml
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Rumen fluid, clarified (see Medium No. 266)	`50.0`	ml
Trypticase peptone (BD-BBL)	`1.0`	g
Vitamin solution (see below)	`5.0`	ml
Butyric acid	`1.7`	g
Na₂SO₄	`2.8`	g
Resazurin	`1.0`	mg
Distilled water	`795.0`	ml

Mix components, adjust pH to 7.2, bring to a boil for a few seconds and cool down under a stream of a N₂-CO₂ (80:20) gas mixture. Distribute Solution A into culture vessels (e.g., 9 ml per tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave.
To complete the medium, add 0.1 volume of 3.5% NaHCO₃ solution [filter-sterilized and stored under a N₂-CO₂ (80:20) gas mixture] and reduce the medium with 0.3 g/L (final) L-cysteine·HCl·H₂O and 0.3 g/L (final) Na₂S·9H₂O (autoclave and store 3% stock solutions under a N₂ atmosphere).
Mineral solution:

KH₂PO₄	`10.0`	g
MgCl₂·6H₂O	`6.6`	g
NaCl	`8.0`	g
NH₄Cl	`8.0`	g
CaCl·2H₂O	`1.0`	g
Distilled water	`1.0`	L

Vitamin solution:

Biotin	`0.25`	mg
Nicotinic acid	`2.5`	mg
Thiamine·HCl	`1.25`	mg
p-Aminobenzoic acid	`1.25`	mg
DL-Calcium pantothenate	`0.62`	mg
Pyridoxine·HCl	`6.2`	mg
Distilled water	`1.0`	L