Medium data

Search for medium no. = [515].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

515   RHODOVULUM VISAKHUM MEDIUM

KH2PO4  0.5     g
MgCl2·6H2O  1.0     g
NaCl  20.0     g
NH4Cl  0.6     g
CaCl2·2H2O  0.15    g
Sorbitol  3.0     g
Sodium pyruvate  3.0     g
Yeast extract  0.4     g
Distilled water  1.0     L
Adjust pH to 6.8.
Add the following components after autoclaving:
Vitamin B12 (20 µg/ml) (filter-sterilized)  1.0     ml
Trace element solution SL8 (see Medium No. 190)  1.0     ml
Adjust pH to 6.8.