Medium data

Search for medium no. = [502].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Solution A:
NaCl  20.0     g
MgCl2·6H2O  3.0     g
Na2SO4  4.0     g
KH2PO4  0.2     g
KCl  0.5     g
NH4Cl  0.25    g
CaCl2·2H2O  0.15    g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
Yeast extract  0.5     g
Vitamin solution (see Medium No. 403)  1.0     ml
Vitamin B12 solution (Medium No. 403)  1.0     ml
Distilled water  860.0     ml
Mix ingredients except Vitamin and Vitamin B12 solutions, adjust pH to 7.0 and autoclave under a N2 gas atmosphere. After autoclaving, add filter-sterilized Vitamin and Vitamin B12 solutions to the solution.
Solution B:
Sodium lactate  2.24    g
Distilled water  100.0     ml
Solution C:
NaHCO3  2.5     g
Distilled water  30.0     ml
Filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture.
Solution D:
Na2S·9H2O  0.36    g
Distilled water  10.0     ml
Autoclave and store under a N2 gas atmosphere.
Separately sterilize the above described solutions. Mix Solutions A, B and C, distribute into culture vessels under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add Solution D and adjust pH to 7.2 - 7.8, if necessary.