Medium data

Search for medium no. = [501].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

501   MODIFIED MARINE MEDIUM FOR DETHIOSULFOBACTER

Solution A:
NaCl  20.0     g
MgCl2·6H2O  3.0     g
Na2SO4  4.0     g
KH2PO4  0.2     g
KCl  0.5     g
NH4Cl  0.25    g
CaCl2·2H2O  0.15    g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
Yeast extract  0.5     g
Vitamin solution (see Medium No. 403)  1.0     ml
Vitamin B12 solution (Medium No. 403)  1.0     ml
Distilled water  760.0     ml
Mix components except Vitamin and Vitamin B12 solutions, adjust pH to 7.0 and autoclave under a N2 gas atmosphere. After autoclaving, add filter-sterilized Vitamin and Vitamin B12 solutions.
Solution B:
Sodium glutamate monohydrate  1.87    g
Distilled water  100.0     ml
Solution C:
Na2S2O3·5H2O  5.0     g
Distilled water  100.0     ml
Solution D:
NaHCO3  2.5     g
Distilled water  30.0     ml
Filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture.
Solution E:
Sodium dithionite  20.0     mg
Distilled water  10.0     ml
Mix components and filter-sterilize just before use.
Separately sterilize the above described solutions. Mix Solutions A, B, C and D, distribute into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Then, add Solution E and adjust pH to 7.2 - 7.8, if necessary.