Medium data

Search for medium no. = [495].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

495   MMJ-DO MEDIUM

Modified MJ synthetic seawater (see Medium No. 492)  1.0     L
Yeast extract  0.1     g
Sodium pyruvate  0.5     g
Sodium lactate  0.5     g
Sodium ascorbate  0.1     g
NaHCO3  1.0     g
Resazurin  1.0     mg
Mix ingredients except NaHCO3, adjust pH to 7.0 with NaOH and autoclave under a H2-CO2 (4:1, v/v) gas mixture. Filter-sterilize 8% NaHCO3 solution and add to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).