Medium data
Search for medium no. = [495].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
495 MMJ-DO MEDIUM
| Modified MJ synthetic seawater (see Medium No. 492) | 1.0 | L |
| Yeast extract | 0.1 | g |
| Sodium pyruvate | 0.5 | g |
| Sodium lactate | 0.5 | g |
| Sodium ascorbate | 0.1 | g |
| NaHCO3 | 1.0 | g |
| Resazurin | 1.0 | mg |
Mix ingredients except NaHCO3, adjust pH to 7.0 with NaOH and autoclave under a H2-CO2 (4:1, v/v) gas mixture. Filter-sterilize 8% NaHCO3 solution and add to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
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