Medium data

Search for medium no. = [494].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

494 MMJ-MH MEDIUM

Modified MJ synthetic seawater (see Medium No. 492)	`1.0`	L
Yeast extract (BD-Difco)	`1.0`	g
Tryptone (BD-Difco)	`1.0`	g
Sulfur (powder)	`3.0`	g
Resazurin	`1.0`	mg
Na₂S·9H₂O	`0.5`	g

Mix ingredients except sulfur and Na₂S·9H₂O, adjust pH to 5.0 and autoclave under a N₂ atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a stream of a H₂-CO₂ (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add 5% Na₂S·9H₂O (autoclaved and stocked under a N₂ atmosphere) to the medium. Readjust pH to 5.0 - 5.3. Pressurize the inoculated bottles to 200 kPa H₂-CO₂ (4:1, v/v).