Medium data
Search for medium no. = [478].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
478 MODIFIED MMJHS MEDIUM FOR GO25
| NaCl | 20.0 | g |
| K2HPO4 | 0.9 | g |
| KH2PO4 | 0.7 | g |
| CaCl2·2H2O | 0.8 | g |
| NH4Cl | 0.25 | g |
| NaNO3 | 1.0 | g |
| MgSO4·7H2O | 4.0 | g |
| MgCl2·6H2O | 3.0 | g |
| KCl | 0.33 | g |
| NiCl2·6H2O | 1.0 | mg |
| Na2SeO3·5H2O | 1.0 | mg |
| Fe2(SO4)3·xH2O | 0.01 | g |
| H2WO4 | 1.0 | mg |
| Na2S2O3·5H2O | 2.0 | g |
| Trace mineral solution (see Medium No. 368) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
| NaHCO3 | 2.0 | g |
| Sulfur (powder) | 10.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix ingredients except Trace vitamins, NaHCO3 and sulfur, adjust pH to 6.8 and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.2 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).
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