Medium data

Search for medium no. = [471].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

471   DESULFONAUTICUS MEDIUM

Use Medium No. 383, replacing sodium lactate with 0.7 g/L sodium acetate and 2.0 g/L yeast extract. Prepare the medium under a H2-CO2 (4:1, v/v) gas atmosphere and reduce the medium with final 0.1 g/L Na2S·9H2O and 0.03 g/L sodium dithionite (filter-sterilized, prepare the solution just before use). Pressurize the inoculated culture vessels to 100 kPa H2-CO2 (4:1, v/v).



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