471 DESULFONAUTICUS MEDIUM
Use Medium No. 383, replacing sodium lactate with 0.7 g/L sodium acetate and 2.0 g/L yeast extract. Prepare the medium under a H2-CO2 (4:1, v/v) gas atmosphere and reduce the medium with final 0.1 g/L Na2S·9H2O and 0.03 g/L sodium dithionite (filter-sterilized, prepare the solution just before use). Pressurize the inoculated culture vessels to 100 kPa H2-CO2 (4:1, v/v).