Medium data
Search for medium no. = [454].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
454 SPIROCHAETA CALIFORNIENSIS MEDIUM
| NaCl | 30.0 | g |
| Na2CO3 | 2.76 | g |
| NaHCO3 | 24.0 | g |
| KCl | 0.2 | g |
| K2HPO4 | 0.2 | g |
| MgCl2·6H2O | 0.1 | g |
| NH4Cl | 1.0 | g |
| Na2S·9H2O | 0.4 | g |
| Yeast extract | 0.5 | g |
| Glucose | 5.0 | g |
| Trace vitamins (see Medium No. 197) | 2.0 | ml |
| Trace minerals solution (see Medium No. 228) | 1.0 | ml |
| Resazurin | 1.0 | mg |
Dissolve components except Na2CO3, NaHCO3 and Na2S·9H2O in 700 ml distilled water and autoclave under a N2 atmosphere in a bottle capped with a butyl rubber stopper. Separately dissolve Na2CO3 and NaHCO3 in 300 ml distilled water, filter-sterilize, and add to the medium aseptically and anaerobically. Adjust pH to 9.0 - 9.5, if necessary. Prior to inoculation, reduce the medium by the addition of a 5% Na2S·9H2O solution autoclaved under a N2 atmosphere.
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