Medium data

Search for medium no. = [454].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

454   SPIROCHAETA CALIFORNIENSIS MEDIUM

NaCl  30.0     g
Na2CO3  2.76    g
NaHCO3  24.0     g
KCl  0.2     g
K2HPO4  0.2     g
MgCl2·6H2O  0.1     g
NH4Cl  1.0     g
Na2S·9H2O  0.4     g
Yeast extract  0.5     g
Glucose  5.0     g
Trace vitamins (see Medium No. 197)  2.0     ml
Trace minerals solution (see Medium No. 228)  1.0     ml
Resazurin  1.0     mg
Dissolve components except Na2CO3, NaHCO3 and Na2S·9H2O in 700 ml distilled water and autoclave under a N2 atmosphere in a bottle capped with a butyl rubber stopper. Separately dissolve Na2CO3 and NaHCO3 in 300 ml distilled water, filter-sterilize, and add to the medium aseptically and anaerobically. Adjust pH to 9.0 - 9.5, if necessary. Prior to inoculation, reduce the medium by the addition of a 5% Na2S·9H2O solution autoclaved under a N2 atmosphere.