Medium data
Search for medium no. = [438].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
438 RT2 MEDIUM
| NaCl | 1.0 | g |
| MgCl2·6H2O | 0.1 | g |
| CaCl2·2H2O | 0.05 | g |
| NH4Cl | 0.25 | g |
| KH2PO4 | 0.1 | g |
| KCl | 0.5 | g |
| Yeast extract | 0.5 | g |
| CAPSO | 11.86 | g |
| Na2SO4 | 4.0 | g |
| Trace element solution (see Medium No. 294) | 1.0 | ml |
| Selenite-tungstate solution (see Medium No. 431) | 1.0 | ml |
| Sodium lactate | 2.24 | g |
| Resazurin | 1.0 | mg |
| Sodium dithionite | 0.1 | g |
Dissolve components except sodium lactate and sodium dithionite in 900 ml distilled water and adjust pH to 9.0 with NaOH. Bring to a boil, and cool down under a N2 gas stream. Distribute the medium into culture vessels (e.g., 9 ml of the medium in Hungate tubes) under N2, seal with butyl rubber stoppers and autoclave. Separately autoclave 0.2 M sodium lactate under a N2 atmosphere. After cooling, add 1/9 volume of the sodium lactate solution to the medium. Readjust pH to 9.0, if necessary. Prior to use, reduce the medium with a freshly prepared, filter-sterilize 1% sodium dithionite solution.
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