Medium data

Search for medium no. = [438].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

438   RT2 MEDIUM

NaCl  1.0     g
MgCl2·6H2O  0.1     g
CaCl2·2H2O  0.05    g
NH4Cl  0.25    g
KH2PO4  0.1     g
KCl  0.5     g
Yeast extract  0.5     g
CAPSO  11.86    g
Na2SO4  4.0     g
Trace element solution (see Medium No. 294)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
Sodium lactate  2.24    g
Resazurin  1.0     mg
Sodium dithionite  0.1     g
Dissolve components except sodium lactate and sodium dithionite in 900 ml distilled water and adjust pH to 9.0 with NaOH. Bring to a boil, and cool down under a N2 gas stream. Distribute the medium into culture vessels (e.g., 9 ml of the medium in Hungate tubes) under N2, seal with butyl rubber stoppers and autoclave. Separately autoclave 0.2 M sodium lactate under a N2 atmosphere. After cooling, add 1/9 volume of the sodium lactate solution to the medium. Readjust pH to 9.0, if necessary. Prior to use, reduce the medium with a freshly prepared, filter-sterilize 1% sodium dithionite solution.

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