Medium data
Search for medium no. = [420].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
420 MJAIS/MD MEDIUM
| NaCl | 30.0 | g |
| K2HPO4 | 0.09 | g |
| KH2PO4 | 0.07 | g |
| CaCl2·2H2O | 0.8 | g |
| NH4Cl | 1.25 | g |
| MgSO4·7H2O | 3.4 | g |
| MgCl2·6H2O | 4.18 | g |
| KCl | 0.33 | g |
| NiCl2·6H2O | 10.0 | mg |
| Na2SeO3·5H2O | 10.0 | mg |
| Na2WO4·2H2O | 10.0 | mg |
| Fe(NH4)2(SO4)2·6H2O | 10.0 | mg |
| FeSO4·7H2O | 20.0 | mg |
| Trace mineral solution (see below) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
| NaHCO3 | 2.0 | g |
| Sulfur (powder) | 30.0 | g |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix ingredients, except Trace vitamins, NaHCO3, sulfur and Na2S·9H2O, adjust pH to 6.0, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S·9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.6 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the sterile Na2S·9H2O solution. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).
Trace mineral solution:
Use Trace mineral solution of Medium No. 324 supplemented with 0.01 g/L SrCl2·6H2O, 0.01 g/L NaBr and 0.01 g/L KI.
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