Medium data

Search for medium no. = [419].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

419   INORGANIC MINERAL MEDIUM WITH THIOSULFATE

NaCl  3.0     g
K2HPO4  0.014   g
CaCl2·2H2O  0.08    g
MgSO4·7H2O  0.34    g
MgCl2·6H2O  0.418   g
KCl  0.33    g
NH4Cl  0.5     g
Na2S2O3·5H2O  1.0     g
Na2SiO3·9H2O  0.8     g
Fe2(SO4)3·xH2O  2.0     mg
FeSO4·7H2O  0.1     mg
NiCl2·6H2O  0.05    mg
Na2SeO3·5H2O  0.05    mg
Trace mineral solution (see Medium No. 324)  1.0     ml
Trace vitamins (see Medium No. 197)  1.0     ml
NaHCO3  0.5     g
Distilled water  1.0     L
Mix ingredients, except Trace vitamins and NaHCO3, and adjust pH to 7.0. Distribute the medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Finally add O2 gas to make 5% (v/v) in the gas phase.