Medium data

Search for medium no. = [419].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

419 INORGANIC MINERAL MEDIUM WITH THIOSULFATE

NaCl	`3.0`	g
K₂HPO₄	`0.014`	g
CaCl₂·2H2O	`0.08`	g
MgSO₄·7H₂O	`0.34`	g
MgCl₂·6H₂O	`0.418`	g
KCl	`0.33`	g
NH₄Cl	`0.5`	g
Na₂S₂O₃·5H₂O	`1.0`	g
Na₂SiO₃·9H₂O	`0.8`	g
Fe₂(SO₄)₃·xH₂O	`2.0`	mg
FeSO₄·7H₂O	`0.1`	mg
NiCl₂·6H₂O	`0.05`	mg
Na₂SeO₃·5H₂O	`0.05`	mg
Trace mineral solution (see Medium No. 324)	`1.0`	ml
Trace vitamins (see Medium No. 197)	`1.0`	ml
NaHCO₃	`0.5`	g
Distilled water	`1.0`	L

Mix ingredients, except Trace vitamins and NaHCO₃, and adjust pH to 7.0. Distribute the medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO₃ solution and add to the medium. Finally add O₂ gas to make 5% (v/v) in the gas phase.