Medium data
Search for medium no. = [416].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
416 MODIFIED MARINE MEDIUM
| NaCl | 19.0 | g |
| MgCl2·6H2O | 9.0 | g |
| MgSO4·7H2O | 0.15 | g |
| CaCl2·2H2O | 0.3 | g |
| KCl | 0.5 | g |
| KH2PO4 | 0.42 | g |
| (NH4)2SO4 | 0.1 | g |
| NaBr | 0.05 | g |
| SrCl2·6H2O | 0.02 | g |
| Yeast extract (BD-Difco) | 0.2 | g |
| Na2SeO4 | 0.02 | g |
| DL-Sodium malate | 1.8 | g |
| KNO3 | 1.0 | g |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Trace mineral solution (see Medium No. 412) | 10.0 | ml |
| L-Cysteine·HCl·H2O | 0.088 | g |
| Distilled water | 1.0 | L |
Mix components, except L-cysteine·HCl·H2O, and adjust pH to 6.5. Distribute the medium into appropriate culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. Separately autoclave concentrated (100- or 200-fold) L-cysteine·HCl·H2O solution under a N2 atmosphere. Prior to inoculation, add the sterile L-cysteine·HCl·H2O solution. Pressurize the inoculated vessels to 100 kPa N2.
Comment: For strain JCM 12418, inoculate with a 10% inoculum.
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