Medium data

Search for medium no. = [391].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

391 MARINE DESULFOSARCINA MEDIUM

NaCl	`20.0`	g
Na₂SO₄	`4.0`	g
MgCl₂·6H₂O	`3.0`	g
KCl	`0.5`	g
CaCl₂·2H₂O	`0.15`	g
NH₄Cl	`0.25`	g
KH₂PO₄	`0.2`	g
Trace element solution (see Medium No. 439)	`1.0`	ml
Resazurin	`1.0`	mg
Distilled water	`970.0`	ml

Mix components thoroughly and adjust pH 7.0. Bring to a boil and cool down under a N₂-CO₂ (4:1, v/v) gas stream. Autoclave under the same gas mixture. After cooling, add the following solutions (filter-sterilized):

Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
8% NaHCO₃ solution	`30.0`	ml
Vitamin solution (see Medium No. 403)	`1.0`	ml
Thiamine solution (see Medium No. 403)	`1.0`	ml
Vitamin B₁₂ solution (see Medium No. 403)	`1.0`	ml

Aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml medium in Balch tubes) and seal with butyl rubber stoppers. Add per liter the following solution (autoclave or *filter-sterilize and store under a N₂ atmosphere):

1.0 M Sodium lactate solution*	`10.0`	ml
5% Na₂S·9H₂O solution	`10.0`	ml

Readjust pH to 7.0 - 7.3, if necessary.
Comment: For strain JCM 31729, replace sodium lactate with final 10 mM sodium fumarate and 0.01% yeast extract.