Medium data
Search for medium no. = [379].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
379 CARNOBACTERIUM PLEISTOCAENIUM MEDIUM
| NaCl | 5.0 | g |
| KH2PO4 | 0.3 | g |
| KCl | 0.3 | g |
| MgSO4·7H2O | 0.1 | g |
| CaSO4·2H2O | 12.5 | mg |
| NH4Cl | 1.0 | g |
| NaHCO3 | 0.4 | g |
| D-Glucose | 5.0 | g |
| Bacto peptone (BD-Difco) | 1.0 | g |
| Yeast extract (BD-Difco) | 0.2 | g |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Trace minerals solution (see Medium No. 228) | 1.0 | ml |
| Resazurin | 1.0 | mg |
| Na2S·9H2O | 0.4 | g |
| Distilled water | 1.0 | L |
Mix ingredients, except NaHCO3 and Na2S·9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of N2 and add NaHCO3 to the medium. Anaerobically dispense the medium into culture vessels, seal with butyl rubber stoppers and autoclave. Separately autoclave 5% Na2S·9H2O solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the Na2S·9H2O solution to the medium. Check final pH to be 7.2-7.4.
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