Medium data

Search for medium no. = [379].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

379   CARNOBACTERIUM PLEISTOCAENIUM MEDIUM

NaCl  5.0     g
KH2PO4  0.3     g
KCl  0.3     g
MgSO4·7H2O  0.1     g
CaSO4·2H2O  12.5     mg
NH4Cl  1.0     g
NaHCO3  0.4     g
D-Glucose  5.0     g
Bacto peptone (BD-Difco)  1.0     g
Yeast extract (BD-Difco)  0.2     g
Trace vitamins (see Medium No. 197)  10.0     ml
Trace minerals solution (see Medium No. 228)  1.0     ml
Resazurin  1.0     mg
Na2S·9H2O  0.4     g
Distilled water  1.0     L
Mix ingredients, except NaHCO3 and Na2S·9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of N2 and add NaHCO3 to the medium. Anaerobically dispense the medium into culture vessels, seal with butyl rubber stoppers and autoclave. Separately autoclave 5% Na2S·9H2O solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the Na2S·9H2O solution to the medium. Check final pH to be 7.2-7.4.



Copyright © 2024 Microbe Division (JCM) - All Rights Reserved