Medium data
Search for medium no. = [378].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
378 ALKALIPHILUS CROTONATOXIDANS MEDIUM
| Yeast extract (BD-Difco) | 2.0 | g |
| Tryptone (BD-Difco) | 2.0 | g |
| Crotonic acid | 0.86 | g |
| KH2PO4 | 0.5 | g |
| MgCl2·6H2O | 0.33 | g |
| NaCl | 0.4 | g |
| NH4Cl | 0.4 | g |
| CaCl2·2H2O | 0.05 | g |
| Trace metal solution (see below) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| NaHCO3 | 3.5 | g |
| L-Cysteine·HCL·H2O | 0.5 | g |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
Dissolve ingredients, except crotonic acid, NaHCO3, L-cysteine·HCl·H2O and Na2S·9H2O, in 900 ml of distilled water and adjust pH to 7.0-7.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense 0.9 volume of the medium into suitable culture vessels under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Add 0.1 volume of a filter-sterilized crotonic acid solution (0.86 g/100 ml, pH 7.0) to the medium. Autoclave cysteine·HCl and Na2S·9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the solutions of crotonic acid, L-cysteine·HCl·H2O and Na2S·9H2O to the medium.
Trace metal solution:
| MnSO4·xH2O | 1.0 | g |
| Fe(NH4)2(SO4)2·6H2O | 0.8 | g |
| CoCl2·6H2O | 0.2 | g |
| ZnSO4·7H2O | 0.2 | g |
| CuCl2·2H2O | 0.02 | g |
| NiCl2·6H2O | 0.02 | g |
| Na2MoO4·2H2O | 0.02 | g |
| Na2SeO4 | 0.02 | g |
| Na2WO4 | 0.02 | g |
| Nitrilotriacetic acid | 2.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 6.0 with KOH.
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