Medium data
Search for medium no. = [373].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
373 VY/2-SWS AGAR
Use Medium No. 341 with the following seawater solution instead of the artificial seawater:
Seawater solution:
| Ferric citrate | 0.1 | g |
| MgSO4·7H2O | 8.0 | g |
| CaCl2·2H2O | 1.0 | g |
| KCl | 0.5 | g |
| NaHCO3 | 0.16 | g |
| H3BO3 | 0.02 | g |
| KBr | 0.08 | g |
| SrCl2·6H2O | 0.03 | g |
| Sodium β-glycerophosphate | 0.01 | g |
| Trace element solution (see below) | 1.0 | ml |
| NaCl | 20.0 | g |
| Distilled water | 1.0 | L |
Trace element solution:
| MnCl2·4H2O | 100.0 | mg |
| CoCl2·6H2O | 20.0 | mg |
| CuSO4·5H2O | 10.0 | mg |
| Na2MoO4·2H2O | 10.0 | mg |
| ZnCl2 | 20.0 | mg |
| LiCl | 5.0 | mg |
| SnCl2·2H2O | 5.0 | mg |
| H3BO3 | 10.0 | mg |
| KBr | 20.0 | mg |
| KI | 20.0 | mg |
| EDTA·Na·Fe(III) | 8.0 | g |
| Distilled water | 1.0 | L |
Copyright © 2025 Microbe Division (JCM) - All Rights Reserved