Medium data

Search for medium no. = [373].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

373   VY/2-SWS AGAR

Use Medium No. 341 with the following seawater solution instead of the artificial seawater:
Seawater solution:
Ferric citrate  0.1     g
MgSO4·7H2O  8.0     g
CaCl2·2H2O  1.0     g
KCl  0.5     g
NaHCO3  0.16    g
H3BO3  0.02    g
KBr  0.08    g
SrCl2·6H2O  0.03    g
Sodium β-glycerophosphate  0.01    g
Trace element solution (see below)  1.0     ml
NaCl  20.0     g
Distilled water  1.0     L
Trace element solution:
MnCl2·4H2O  100.0     mg
CoCl2·6H2O  20.0     mg
CuSO4·5H2O  10.0     mg
Na2MoO4·2H2O  10.0     mg
ZnCl2  20.0     mg
LiCl  5.0     mg
SnCl2·2H2O  5.0     mg
H3BO3  10.0     mg
KBr  20.0     mg
KI  20.0     mg
EDTA·Na·Fe(III)  8.0     g
Distilled water  1.0     L