Medium data

Search for medium no. = [341].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

341   VY/2-ASW AGAR

Yeast cell paste (see below)  5.0     g
Vitamin B12  0.5     mg
Agar  15.0     g
Artificial seawater  1.0     L
Adjust pH to 7.5. Autoclave at 120C for 20 min. Separately sterilize vitamin B12 by filtration, and aseptically add to the medium.
Yeast cell paste:
Wash dried yeast (e.g., Yeast from Saccharomyces cerevisiae Type II, Sigma) with distilled water three times.