Medium data
Search for medium no. = [369].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
369 MMJH MEDIUM
NaCl | 30.0 | g |
K2HPO4 | 0.14 | g |
CaCl2·2H2O | 1.0 | g |
NH4Cl | 0.25 | g |
NaNO3 | 1.0 | g |
MgSO4·7H2O | 3.4 | g |
MgCl2·6H2O | 4.18 | g |
KCl | 0.33 | g |
NiCl2·6H2O | 5.0 | mg |
Na2SeO3·5H2O | 0.5 | mg |
Fe2(SO4)3·xH2O | 0.05 | g |
H2WO4 | 5.0 | mg |
Trace mineral solution (see below) | 10.0 | ml |
Trace vitamins (see Medium No. 197) | 1.0 | ml |
NaHCO3 | 1.0 | g |
Distilled water | 1.0 | L |
Mix ingredients except Fe2(SO4)3·xH2O, Trace vitamins and NaHCO3, adjust pH to 6.8 and autoclave. Separately autoclave 0.5% Fe2(SO4)3·xH2O solution (in 0.1 N H2SO4), filter-sterilize Trace vitamins and 5% NaHCO3 solution and add the solutions to the medium. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2-O2 (80:20:1, v/v) gas mixture and seal with butyl rubber stoppers. Readjust pH to 6.8, if necessary. Pressurize the inoculated bottle to 200 kPa with the same gas mixture.
Trace mineral solution:
Use Trace mineral solution of Medium No. 324 supplemented with 0.01 g/L SrCl2·H2O, 0.01 g/L NaBr and 0.01 g/L KI.
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