Medium data

Search for medium no. = [369].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

369   MMJH MEDIUM

NaCl  30.0     g
K2HPO4  0.14    g
CaCl2·2H2O  1.0     g
NH4Cl  0.25    g
NaNO3  1.0     g
MgSO4·7H2O  3.4     g
MgCl2·6H2O  4.18    g
KCl  0.33    g
NiCl2·6H2O  5.0     mg
Na2SeO3·5H2O  0.5     mg
Fe2(SO4)3·xH2O  0.05    g
H2WO4  5.0     mg
Trace mineral solution (see below)  10.0     ml
Trace vitamins (see Medium No. 197)  1.0     ml
NaHCO3  1.0     g
Distilled water  1.0     L
Mix ingredients except Fe2(SO4)3·xH2O, Trace vitamins and NaHCO3, adjust pH to 6.8 and autoclave. Separately autoclave 0.5% Fe2(SO4)3·xH2O solution (in 0.1 N H2SO4), filter-sterilize Trace vitamins and 5% NaHCO3 solution and add the solutions to the medium. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2-O2 (80:20:1, v/v) gas mixture and seal with butyl rubber stoppers. Readjust pH to 6.8, if necessary. Pressurize the inoculated bottle to 200 kPa with the same gas mixture.
Trace mineral solution:
Use Trace mineral solution of Medium No. 324 supplemented with 0.01 g/L SrCl2·H2O, 0.01 g/L NaBr and 0.01 g/L KI.



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