Medium data

Search for medium no. = [363].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

363   PY4S AGAR

Salt solution I (see Medium No. 133)  75.0     ml
Salt solution II (see Medium No. 133)  75.0     ml
Trypticase peptone (BD-BBL)  10.0     g
Yeast extract (BD-Difco)  5.0     g
Glucose  0.25    g
Cellobiose  0.25    g
Maltose  0.25    g
Soluble starch  0.25    g
0.1% (w/v) resazurin-Na  1.0     ml
8% (w/v) Na2CO3  2.5     ml
L-Cysteine·HCl·H2O  0.3     g
Bacto agar (BD-Difco)  15.0     g
Distilled water  850.0     ml
Adjust pH to 6.7. Prepare medium anaerobically and tube in rubber stopped tubes under oxygen-free N2-CO2 (95:5).