Medium data

Search for medium no. = [316].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NaCl  256.0     mg
KH2PO4  1.7     mg
CaCl2·2H2O  0.8     mg
H3BO3  10.3     mg
Na2SO4  23.0     mg
NaNO3  0.3     mg
KCl  15.0     mg
FeCl3·6H2O  0.1     mg
MnSO4·xH2O  0.006   mg
Trace minerals (see Medium No. 151)  10.0     ml
(NH4)2Ni(SO4)2·6H2O  2.0     mg
Na2WO4·2H2O  0.1     mg
Na2SeO4  0.1     mg
NaHCO3  1.0     g
Na2S2O3·5H2O  1.0     g
Distilled water  1.0     L
Mix ingredients except Na2S2O3·5H2O, distribute the medium to culture vessels under a N2 gas atmosphere, and seal with butyl rubber stoppers. Autoclave and stand the culture vessels overnight. Add filter-sterilized 10% Na2S2O3·5H2O solution and adjust pH to 7.0 with 1 N H2SO4. Replace the gas phase with a N2-O2-H2 (96:1:3, v/v) gas mixture, and pressurized the inoculated bottles to 300 kPa.