Medium data
Search for medium no. = [316].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
316 THERMOCRINIS ALBUS MEDIUM
| NaCl | 256.0 | mg |
| KH2PO4 | 1.7 | mg |
| CaCl2·2H2O | 0.8 | mg |
| H3BO3 | 10.3 | mg |
| Na2SO4 | 23.0 | mg |
| NaNO3 | 0.3 | mg |
| KCl | 15.0 | mg |
| FeCl3·6H2O | 0.1 | mg |
| MnSO4·xH2O | 0.006 | mg |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| (NH4)2Ni(SO4)2·6H2O | 2.0 | mg |
| Na2WO4·2H2O | 0.1 | mg |
| Na2SeO4 | 0.1 | mg |
| NaHCO3 | 1.0 | g |
| Na2S2O3·5H2O | 1.0 | g |
| Distilled water | 1.0 | L |
Mix ingredients except Na2S2O3·5H2O, distribute the medium to culture vessels under a N2 gas atmosphere, and seal with butyl rubber stoppers. Autoclave and stand the culture vessels overnight. Add filter-sterilized 10% Na2S2O3·5H2O solution and adjust pH to 7.0 with 1 N H2SO4. Replace the gas phase with a N2-O2-H2 (96:1:3, v/v) gas mixture, and pressurized the inoculated bottles to 200 kPa.
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