Medium data

Search for medium no. = [315].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

315

K₂HPO₄	`0.78`	g
KH₂PO₄	`0.75`	g
EDTA·3Na	`0.04`	g
FeSO₄·7H₂O	`0.01`	g
MgSO₄·7H₂O	`0.25`	g
CaCl₂·2H₂O	`0.03`	g
NaCl	`0.2`	g
NH₄Cl	`0.5`	g
Glucose	`0.5`	g
Yeast extract (BD-Difco)	`0.5`	g
Na₂S₂O₃·5H₂O	`1.24`	g
Trace vitamins (see Medium No. 197)	`10.0`	ml
Trace mineral solution (see Medium No. 197)	`10.0`	ml
NaHCO₃	`5.0`	g
L-Cysteine·HCl·H₂O	`0.5`	g
Distilled water	`1.0`	L

Mix ingredients except cysteine·HCl·H₂O, NaHCO₃ and Trace vitamins, and autoclave under a N₂-CO₂ (80:20) atmosphere. Filter-sterilize NaHCO₃ as a 10% solution and Trace vitamins while gassing with CO₂. Separately autoclave L-cysteine·HCl·H₂O as a 5% solution under a CO₂ atmosphere. Aseptically and anaerobically add NaHCO₃ and Trace vitamins to the medium under a N₂-CO₂ (80:20) atmosphere. Prior to inoculation, add the sterile L-cysteine·HCl·H₂O solution.