Medium data

Search for medium no. = [314].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

314   

Na2CO3  90.0     g
NaHCO3  20.0     g
NaCl  12.0     g
2 M Na2S2O3 solution  20.0     ml
1 M KNO3 solution  5.0     ml
20% MgCl2·6H2O solution  1.0     ml
Trace elements solution (see Medium No. 310)  1.0     ml
Distilled water  1.0     L
Filter-sterilize Na2S2O3 solution and KNO3 solution, and separately autoclave Trace elements solution and MgCl2·6H2O solution. Aseptically add Na2S2O3 solution, KNO3 solution, Trace elements solution and MgCl2·6H2O solution to the medium.