Medium data

Search for medium no. = [314].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

314

Na₂CO₃	`90.0`	g
NaHCO₃	`20.0`	g
NaCl	`12.0`	g
2 M Na₂S₂O₃ solution	`20.0`	ml
1 M KNO₃ solution	`5.0`	ml
20% MgCl₂·6H₂O solution	`1.0`	ml
Trace elements solution (see Medium No. 310)	`1.0`	ml
Distilled water	`1.0`	L

Filter-sterilize Na₂S₂O₃ solution and KNO₃ solution, and separately autoclave Trace elements solution and MgCl₂·6H₂O solution. Aseptically add Na₂S₂O₃ solution, KNO₃ solution, Trace elements solution and MgCl₂·6H₂O solution to the medium.