Medium data
Search for medium no. = [310].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
310
| Na2CO3 | 20.0 | g |
| NaHCO3 | 10.0 | g |
| NaCl | 5.0 | g |
| K2HPO4 | 1.0 | g |
| 2 M KSCN solution | 7.5 | ml |
| 20% MgCl2·6H2O solution | 1.0 | ml |
| Trace elements solution (see below) | 1.0 | ml |
| Distilled water | 1.0 | L |
Filter-sterilize KSCN solution and separately autoclave Trace elements solution and MgCl2·6H2O solution. Aseptically add KSCN solution, Trace elements solution and MgCl2·6H2O solution to the medium.
Trace elements solution:
| EDTA | 5.0 | g |
| FeSO4·7H2O | 2.0 | g |
| ZnSO4·7H2O | 0.1 | g |
| MnCl2·4H2O | 0.03 | g |
| CoCl2·6H2O | 0.2 | g |
| NiCl2·6H2O | 0.02 | g |
| Na2MoO4·2H2O | 0.03 | g |
| CuCl2·2H2O | 0.01 | g |
| H3BO3 | 0.3 | g |
| Distilled water | 1.0 | L |
Adjust pH to 3 with HCl.
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