Medium data

Search for medium no. = [286].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

286   HYDROGENOTHERMUS MEDIUM

Use Aquifex medium (Medium No. 255) without Trace vitamins and supplement with 0.2 g/L yeast extract (BD-Difco) and 0.5 g/L sulfur (powder). Mix ingredients except sulfur and adjust pH to 7.0 with H2SO4. Autoclave the medium under a gas mixture of H2-CO2 (4:1, v/v), and stand overnight. Steam sulfur for 3 hr on each of 3 successive days. Aseptically dispense 20 ml of the medium into serum bottles (120 ml) containing the sterilized sulfur (0.01 g/bottle) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. After inoculation, add 15.0 ml filter-sterilized air, and pressurize to 200 kPa with a H2-CO2 (4:1, v/v) gas mixture.



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