Medium data
Search for medium no. = [279].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
279 HYDROGENOPHILUS HIRSCHII MEDIUM
| MgSO4·7H2O | 7.0 | g |
| MgCl2·6H2O | 5.5 | g |
| KCl | 0.65 | g |
| NaBr | 0.1 | g |
| NaHCO3 | 2.0 | g |
| NH4Cl | 0.15 | g |
| CaCl2·2H2O | 0.5 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| (NH4)2Ni(SO4)2·6H2O | 0.02 | g |
| Na2WO4·2H2O | 0.1 | mg |
| Na2SeO4 | 0.1 | mg |
| Na2S2O3·5H2O | 1.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 7.0 with H2SO4. Distribute 20 ml of medium into 120 ml serum bottles under a stream of a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Autoclave and stand the bottles overnight. Add 15 ml filter-sterilized air and pressurize the gas phase to 200 kPa with a H2-CO2 (4:1, v/v) gas mixture.
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