Medium data
Search for medium no. = [277].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
277 MJYP* MEDIUM
Yeast extract (BD-Difco) | 2.0 | g |
Trypticase peptone (BD-BBL) | 2.0 | g |
NaNO3 | 1.70 | g |
FeSO4·7H2O | 1.39 | g |
Na2S·9H2O | 0.5 | g |
Resazurin | 1.0 | mg |
MJ(-N) synthetic seawater (see Medium No. 268) | 1.0 | L |
Mix ingredients, except NaNO3, FeSO4·7H2O and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave NaNO3, FeSO4·7H2O and Na2S·9H2O solutions (pH 7.0, 7.5 and 7.5, respectively) under a N2 atmosphere. Aseptically and anaerobically add the NaNO3, FeSO4·7H2O and Na2S·9H2O solutions to the medium and adjust pH to 6.0. Pressurize the inoculated bottle to 200 kPa N2.
Copyright © 2025 Microbe Division (JCM) - All Rights Reserved