Medium data

Search for medium no. = [277].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

277   MJYP* MEDIUM

Yeast extract (BD-Difco)  2.0     g
Trypticase peptone (BD-BBL)  2.0     g
NaNO3  1.70    g
FeSO4·7H2O  1.39    g
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
MJ(-N) synthetic seawater (see Medium No. 268)  1.0     L
Mix ingredients, except NaNO3, FeSO4·7H2O and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave NaNO3, FeSO4·7H2O and Na2S·9H2O solutions (pH 7.0, 7.5 and 7.5, respectively) under a N2 atmosphere. Aseptically and anaerobically add the NaNO3, FeSO4·7H2O and Na2S·9H2O solutions to the medium and adjust pH to 6.0. Pressurize the inoculated bottle to 200 kPa N2.



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