Medium data

Search for medium no. = [274].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

274   MJ/YTCT MEDIUM

Yeast extract (BD-Difco)  2.0     g
Tryptone (BD-Difco)  2.0     g
Sodium tartrate  1.0     g
Trisodium citrate  1.0     g
NaNO3  0.25    g
(NH4)2SO4  0.25    g
Ferric citrate  0.2     g
MJ(-N) synthetic seawater (see Medium No. 268)  100.0     ml
Na2SO4  1.0     g
Na2S2O3·5H2O  2.0     g
Na2CO3  40.0     g
Distilled water  900.0     ml
Adjust pH to 11.0-12.0 with KOH and autoclave under a N2 atmosphere. After autoclaving, replace the gas phase with N2-CO2 (80:20) and pressurize to 200 kPa.