Medium data
Search for medium no. = [274].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
274 MJ/YTCT MEDIUM
Yeast extract (BD-Difco) | 2.0 | g |
Tryptone (BD-Difco) | 2.0 | g |
Sodium tartrate | 1.0 | g |
Trisodium citrate | 1.0 | g |
NaNO3 | 0.25 | g |
(NH4)2SO4 | 0.25 | g |
Ferric citrate | 0.2 | g |
MJ(-N) synthetic seawater (see Medium No. 268) | 100.0 | ml |
Na2SO4 | 1.0 | g |
Na2S2O3·5H2O | 2.0 | g |
Na2CO3 | 40.0 | g |
Distilled water | 900.0 | ml |
Adjust pH to 11.0-12.0 with KOH and autoclave under a N2 atmosphere. After autoclaving, replace the gas phase with N2-CO2 (80:20) and pressurize to 200 kPa.
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