Medium data
Search for medium no. = [270].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
270 GNYS MEDIUM
| Sodium gluconate | 3.0 | g |
| Yeast extract (BD-Difco) | 1.0 | g |
| (NH4)2SO4 | 1.0 | g |
| KH2PO4 | 1.0 | g |
| NaCl | 0.2 | g |
| MgCl2·6H2O | 0.2 | g |
| CaCl2·2H2O | 0.05 | g |
| Trace element solution SL8 (see Medium No. 190) | 1.0 | ml |
| Distilled water | 1.0 | L |
Adjust pH to 4.0-4.5.
For preparation of solid medium, add 18.0 g/L agar and adjust pH to 5.0 to avoid hydrolysis of agar.
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