Medium data
Search for medium no. = [237].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
237 THERMOTOGA MEDIUM
| Soluble starch | 5.0 | g |
| KH2PO4 | 0.5 | g |
| Trace minerals (see Medium No. 151) | 15.0 | ml |
| NiCl2·6H2O | 2.0 | mg |
| NaCl | 20.0 | g |
| Yeast extract (BD-Difco) | 0.5 | g |
| Resazurin | 1.0 | mg |
| Na2S·9H2O | 0.5 | g |
| Artificial seawater (see below) | 250.0 | ml |
| Distilled water | 750.0 | ml |
Mix components except Na2S·9H2O, adjust pH to 6.5 and autoclave under a N2 atmosphere. Separately autoclave a 5% Na2S·9H2O solution (pH 7.0) under a N2 atmosphere. Prior to inoculation, add the sterile Na2S·9H2O solution to the medium.
Artificial seawater:
| NaCl | 27.7 | g |
| MgSO4·7H2O | 7.0 | g |
| MgCl2·6H2O | 5.5 | g |
| KCl | 0.65 | g |
| NaBr | 0.1 | g |
| H3BO3 | 30.0 | mg |
| SrCl2·6H2O | 15.0 | mg |
| Citric acid | 10.0 | mg |
| KI | 0.05 | mg |
| CaCl2·2H2O | 2.25 | g |
| Distilled water | 1.0 | L |
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