Medium data
Search for medium no. = [215].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
215 PYROBACULUM AEROPHILUM MEDIUM
| Marine medium/Synthetic seawater mix solution (see below) | 125.0 | ml |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Fe(NH4)2(SO4)2·6H2O | 2.0 | mg |
| NH4Cl | 0.25 | g |
| (NH4)2Ni(SO4)2·6H2O | 2.0 | mg |
| Na2SeO4 | 0.1 | mg |
| NaWO4·2H2O | 0.1 | mg |
| NaHCO3 | 2.2 | g |
| KH2PO4 | 0.07 | g |
| Yeast extract (BD-Difco) | 0.5 | g |
| Na2S2O3·5H2O | 1.0 | g |
| Distilled water | 865.0 | ml |
Adjust pH to 7.0 with 1 N H2SO4, and sterilize by filtration. Dispense the medium into bottles under a N2-CO2-O2 (80:20:1) atmosphere. Pressurize inoculated bottles to 200 kPa N2-CO2-O2 (80:20:1).
Marine medium/Synthetic seawater mix solution:
| NaCl | 47.15 | g |
| MgCl2·6H2O | 18.10 | g |
| MgSO4·7H2O | 7.0 | g |
| CaCl2·2H2O | 3.13 | g |
| Na2SO4 | 3.24 | g |
| Na2CO3 | 0.1 | g |
| NaBr | 0.1 | g |
| KBr | 80.0 | mg |
| SrCl2·6H2O | 72.0 | mg |
| H3BO3 | 52.0 | mg |
| Na2HPO4 | 8.1 | mg |
| NaF | 2.4 | mg |
| Sodium silicate | 0.4 | mg |
| KI | 0.05 | mg |
| Distilled water | 1.0 | L |
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