Medium data
Search for medium no. = [214].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
214 ARCHAEOGLOBUS PROFUNDUS MEDIUM
| KCl | 0.34 | g |
| MgCl2·6H2O | 4.0 | g |
| MgSO4·7H2O | 3.45 | g |
| NH4Cl | 0.25 | g |
| CaCl2·2H2O | 0.14 | g |
| K2HPO4 | 0.14 | g |
| NaCl | 18.0 | g |
| NaHCO3 | 1.0 | g |
| Na2SO4 | 2.7 | g |
| Fe(NH4)2(SO4)2·6H2O | 2.0 | mg |
| (NH4)2Ni(SO4)2·6H2O | 2.0 | mg |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Yeast extract (BD-Difco) | 0.5 | g |
| Sodium acetate | 1.0 | g |
| Resazurin | 1.0 | mg |
| Na2S·9H2O | 0.5 | g |
| Distilled water | 990.0 | ml |
Mix ingredients except Na2S·9H2O and adjust pH to 6.9 with H2SO4. Sterilize by filtration under H2-CO2 (80:20) gas mixture. Neutralize Na2S·9H2O solution and autoclave under a N2 atmosphere. Aseptically and anaerobically add the sterile Na2S·9H2O solution to the medium under a H2-CO2 (80:20) atmosphere and readjust pH to 6.9, if necessary. Pressurize inoculated bottle to 200 kPa H2-CO2 (80:20).
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