Medium data

Search for medium no. = [213].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


KCl  0.34    g
MgCl2·6H2O  4.0     g
MgSO4·7H2O  3.45    g
NH4Cl  0.25    g
CaCl2·2H2O  0.14    g
K2HPO4  0.14    g
NaCl  18.0     g
NaHCO3  5.0     g
Fe(NH4)2(SO4)2·6H2O  2.0     mg
(NH4)2Ni(SO4)2·6H2O  2.0     mg
Trace minerals (see Medium No. 151)  10.0     ml
Yeast extract (BD-Difco)  0.5     g
Sodium L-lactate  1.5     g
Resazurin  1.0     mg
Na2S·9H2O  0.5     g
Distilled water  990.0     ml
Mix ingredients, except Na2S·9H2O, and adjust pH to 6.9 with 1 N H2SO4. Sterilize by filtration under a N2-CO2 (80:20) gas mixture. Neutralize Na2S·9H2O as a 5% solution and autoclave under a N2 atmosphere. Aseptically and anaerobically add the sterile Na2S·9H2O solution to the medium under a N2-CO2 (80:20) atmosphere, and readjust pH to 6.9, if necessary. Pressurize inoculated bottles to 200 kPa N2-CO2 (80:20).