Medium data

Search for medium no. = [206].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Salt base solution (see below)  1.0     L
Trace minerals (see Medium No. 151)  10.0     ml
Citric acid  5.0     mg
Tryptone (BD-Difco)  6.0     g
Sulfur (powder)  6.0     g
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
Mix ingredients, except sulfur and Na2S·9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration while gassing with H2-CO2 (80:20) gas mixture. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S·9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S·9H2O solution. Pressurize inoculated bottles to 100 kPa H2-CO2 (80:20).
Salt base solution:
Use Salt base solution of Medium No. 151 with 17.0 g/L (final) NaCl and 2.5 mg/L (final) KI.