Medium data
Search for medium no. = [203].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
203 PYRODICTIUM BROCKII MEDIUM
| Salt base solution (see Medium No. 151) | 1.0 | L |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Yeast extract (BD-Difco) | 2.0 | g |
| Sulfur (powder) | 30.0 | g |
| Resazurin | 1.0 | mg |
Mix components except sulfur, adjust pH to 5.5 with H2SO4 and autoclave under a N2 gas atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium and sulfur into culture vessels (e.g., 20 ml of the medium and 0.6 g sulfur in 120 ml serum bottles) under a stream of a H2-CO2 (80:20, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add per litter the following solution (autoclave and stored under a N2 atmosphere):
| 5% Na2S·9H2O solution (pH 7.0) | 10.0 | ml |
Readjust pH to 5.5, if necessary. Pressurize inoculated vessels to 200kPa H2 -CO2 (80:20, v/v).
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