Medium data
Search for medium no. = [198].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
198
| KH2PO4 | 0.75 | g |
| K2HPO4 | 0.75 | g |
| MnCl2·4H2O | 0.36 | g |
| NH4Cl | 1.0 | g |
| NaHCO3 | 4.0 | g |
| Trace mineral solution (see Medium No. 197) | 9.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Na2S·9H2O | 0.5 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix ingredients, except NaHCO3, Trace vitamins, Na2S·9H2O and L-cysteine·HCl·H2O, and autoclave under a H2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S·9H2O and L-cysteine·HCl·H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add the NaHCO3 solution and Trace vitamins to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add sterile the Na2S·9H2O and L-cysteine·HCl solutions, and adjust pH to 7.4. Pressurize inoculated bottles to 200 kPa H2-CO2 (80:20).
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