Medium data

Search for medium no. = [198].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

198   

KH2PO4  0.75    g
K2HPO4  0.75    g
MnCl2·4H2O  0.36    g
NH4Cl  1.0     g
NaHCO3  4.0     g
Trace mineral solution (see Medium No. 197)  9.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Na2S·9H2O  0.5     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix ingredients, except NaHCO3, Trace vitamins, Na2S·9H2O and L-cysteine·HCl·H2O, and autoclave under a H2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S·9H2O and L-cysteine·HCl·H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add the NaHCO3 solution and Trace vitamins to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add sterile the Na2S·9H2O and L-cysteine·HCl solutions, and adjust pH to 7.4. Pressurize inoculated bottles to 200 kPa H2-CO2 (80:20).