Medium data

Search for medium no. = [194].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

194   MMYS-II MEDIUM

Solution I:
(NH4)2SO4  1.0     g
NaCl  8.2     g
MgCl2·6H2O  0.2     g
CaCl2·2H2O  0.05    g
Trace element solution SL8 (see Medium No. 190)  1.0     ml
Sodium DL-malate  3.6     g
Yeast extract  1.0     g
Na2S2O3·5H2O  0.5     g
Distilled water  500.0     ml
Solution II:
20 mM Potassium phosphate buffer (pH 7.8)  500.0     ml
Separately autoclave Solutions I and II. Mix aseptically and adjust final pH to 7.7-7.8.