Medium data
Search for medium no. = [194].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
194 MMYS-II MEDIUM
Solution I:
| (NH4)2SO4 | 1.0 | g |
| NaCl | 8.2 | g |
| MgCl2·6H2O | 0.2 | g |
| CaCl2·2H2O | 0.05 | g |
| Trace element solution SL8 (see Medium No. 190) | 1.0 | ml |
| Sodium DL-malate | 3.6 | g |
| Yeast extract | 1.0 | g |
| Na2S2O3·5H2O | 0.5 | g |
| Distilled water | 500.0 | ml |
Solution II:
| 20 mM Potassium phosphate buffer (pH 7.8) | 500.0 | ml |
Separately autoclave Solutions I and II. Mix aseptically and adjust final pH to 7.7-7.8.
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