Medium data

Search for medium no. = [192].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

192 PYS MEDIUM

KH₂PO₄	`1.0`	g
(NH₄)₂SO₄	`1.0`	g
NaCl	`0.2`	g
MgCl₂·6H₂O	`0.2`	g
CaCl₂·2H₂O	`0.05`	g
Trace element solution SL8 (see Medium No. 190)	`1.0`	ml
Sodium pyruvate	`2.2`	g
Yeast extract	`1.0`	g
Distilled water	`1.0`	L

Adjust pH to 6.8.
Separately sterilize sodium pyruvate by filtration, and aseptically add to the medium.