Medium data

Search for medium no. = [175].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Modified Brock's salt base solution (see Medium No. 165)  1.0     L
Yeast extract (BD-Difco)  0.5     g
Sulfur (powder)  1.0     g
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
Mix ingredients except yeast extract, sulfur and Na2S·9H2O, and autoclave under a H2-CO2 (80:20) atmosphere. Separately autoclave yeast extract as a 10% solution and Na2S·9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add the yeast extract solution and sulfur to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S·9H2O solution and adjust pH to 2.5 with sterile 1 N H2SO4. Pressurize inoculated bottles to 100 kPa H2-CO2 (80:20).

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