Medium data

Search for medium no. = [165].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

165   SULFOLOBUS MEDIUM

Modified Brock's salt base solution (see below)  1.0     L
Yeast extract (BD-Difco)  1.0     g
Adjust pH to 2.0 with 10 N H2SO4.
Modified Brock's salt base solution:
(NH4)2SO4  1.3     g
KH2PO4  0.28    g
MgSO4·7H2O  0.25    g
CaCl2·2H2O  0.07    g
FeCl3·6H2O  2.0     mg
MnCl2·4H2O  1.8     mg
Na2B4O7·10H2O  4.5     mg
ZnSO4·7H2O  0.22    mg
CuCl2·2H2O  0.05    mg
Na2MoO4·2H2O  0.03    mg
VOSO4·xH2O  0.03    mg
CoSO4·7H2O  0.01    mg
Distilled water  1.0     L