Medium data
Search for medium no. = [174].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
174 ACIDIANUS INFERNUS MEDIUM (A)
| Modified Brock's salt base solution (see Medium No. 165) | 1.0 | L |
| Yeast extract (BD-Difco) | 0.5 | g |
| Sulfur (powder) | 1.0 | g |
Adjust pH to 2.5 with 10 N H2SO4. Autoclave yeast extract separately and steam sulfur for 3 hr on each of 3 successive days. Cultivate under air enriched with 1-10% CO2.
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