Medium data

Search for medium no. = [174].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

174   ACIDIANUS INFERNUS MEDIUM (A)

Modified Brock's salt base solution (see Medium No. 165)  1.0     L
Yeast extract (BD-Difco)  0.5     g
Sulfur (powder)  1.0     g
Adjust pH to 2.5 with 10 N H2SO4. Autoclave yeast extract separately and steam sulfur for 3 hr on each of 3 successive days. Cultivate under air enriched with 1-10% CO2.