Medium data

Search for medium no. = [160].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

160   THERMOCOCCUS STETTERI MEDIUM

Modified Brock's salt base solution (see Medium No. 165)  1.0     L
NaCl  25.0     g
Yeast extract (BD-Difco)  3.0     g
Bacto peptone (BD-Difco)  0.5     g
Sulfur (powder)  10.0     g
Resazurin  1.0     mg
Mix ingredients, except yeast extract, sulfur and Na2S·9H2O, adjust pH to 6.5 with H2SO4, and autoclave under a N2 atmosphere. Separately autoclave yeast extract and peptone as 10% solutions under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S·9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add the yeast extract and peptone solutions, and sulfur to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution, and readjust pH to 6.5, if necessary.