Medium data
Search for medium no. = [159].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
159 THERMOCOCCUS CELER MEDIUM
| Modified Brock's salt base solution (see Medium No. 165) | 1.0 | L |
| NaCl | 40.0 | g |
| Yeast extract (BD-Difco) | 2.0 | g |
| Sulfur (powder) | 10.0 | g |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
Mix ingredients, except yeast extract, sulfur and Na2S·9H2O, adjust pH to 5.8 with H2SO4, and autoclave under a N2 atmosphere. Separately autoclave yeast extract as a 10% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S·9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add the yeast extract solution and sulfur to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution, and readjust pH to 5.8, if necessary.
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