Medium data

Search for medium no. = [1445].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1445 PMX.108 MEDIUM

NH₄Cl	`0.54`	g
KH₂PO₄	`0.14`	g
MgCl₂•6H₂O	`0.2`	g
CaCl₂•2H₂O	`0.15`	g
Sodium aceate•3H₂O	`1.36`	g
Yeast extract	`0.09`	g
Casamino acids (BD-Difico)	`0.5`	g
Tryptone (BD-Difico)	`1.5`	g
Trace mineral solution (see Medium No. 852)	`1.0`	ml
Se/W solution (see below)	`1.0`	ml
Trace vitamins solution (see Medium No. 284)	`2.0`	ml
Resazurin	`1.0`	mg
Distilled water	`970.0`	ml

Mix components thoroughly, bring to a boil, and cool down under a N₂-CO₂ (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture (e. g., 20 ml medium in 60 ml serum bottles), seal with butyl rubber stoppers and inject 10-20% gas-phase volume of H₂, and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):

8% NaHCO₃ solution*	`30.0`	ml
5% L-Cysteine•HCl•H₂O solution	`4.0`	m
5% Na₂S•9H₂O solution	`4.0`	m

Se/W solution:

Na₂SeO₃	`2.0`	mg
Na₂WO₄•2H₂O	`1.0`	mg
Distilled water	`1.0`	L