Medium data

Search for medium no. = [1436].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1436 LIMNOCHORDIA L945 MEDIUM

KH₂PO₄	`0.2`	g
MgCl₂·6H₂O	`0.04`	g
CaCl₂·2H₂O	`0.1`	g
KCl	`0.5`	g
NH₄Cl	`0.25`	g
Wolfe’s mineral solution (see Medium No. 265)	`10.0`	ml
Resazurin	`0.5`	mg
Distilled water	`890.0`	ml

Mix components thoroughly, sparge the medium with a N₂-CO₂ (80:20, v/v) gas mixture and autoclave under the same gas mixture. After cooling, aseptically and anaerobically add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):

8% NaHCO₃ solution*	`60.0`	ml
10% Glucose solution	`20.0`	ml
1.0 M Sodium fumarate solution	`10.0`	ml
Trace vitamins* (see Medium No. 197)	`10.0`	ml

Prior to use, add per liter the following solution (autoclave and store under a N₂ atmosphere):

5% Na₂S·9H₂O solution 10.0 ml

Adjust pH to 7.5, if necessary.