Medium data
Search for medium no. = [1435].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1435 LIMNOCHORDIA LN MEDIUM
| K2HPO4 | 0.78 | g |
| KH2PO4 | 0.7 | g |
| EDTA•2Na | 0.04 | g |
| FeSO4·7H2O | 0.01 | g |
| MgSO4·7H2O | 0.25 | g |
| CaCl2·2H2O | 0.1 | g |
| NaCl | 0.2 | g |
| Trace element solution (see below) | 10.0 | ml |
| Resazurin | 0.5 | mg |
| Distilled water | 940.0 | ml |
Mix components thoroughly, sparge the medium with a N2-CO2 (80:20, v/v) gas mixture and autoclave under the same gas mixture. After cooling, aseptically and anaerobically add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 25.0 | ml |
| 10% Glucose solution | 5.0 | ml |
| 10% Yeast extract (Oxoid) solution | 5.0 | ml |
| 1.0 M Sodium thiosulfate solution | 5.0 | ml |
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
| 5% L-Cysteine·HCl·H2O solution | 5.0 | ml |
Adjust pH to 6.5, if necessary.
Trace element solution:
| Nitrilotriacetic acid | 12.8 | g |
| FeCl2·4H2O | 1.0 | g |
| MnCl2·4H2O | 0.1 | g |
| CoCl2·6H2O | 0.03 | g |
| CaCl2·2H2O | 0.1 | g |
| ZnCl2 | 0.1 | g |
| CuCl2·2H2O | 0.02 | g |
| H3BO3 | 0.01 | g |
| Na2MoO4·2H2O | 0.03 | g |
| NiCl2·6H2O | 0.1 | g |
| NaCl | 1.0 | g |
| Na2SeO3 | 0.02 | g |
| Na2WO4·2H2O | 0.04 | g |
| Distilled water | 1.0 | L |
Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH 6.5 with KOH. Then, add the remaining salts and adjust volume to 1000 ml. Readjust pH to 6.5.
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