Medium data
Search for medium no. = [1429].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1429 M1H NAG ASW
| HEPES | 23.8 | g |
| Peptone | 0.25 | g |
| Yeast extract (BD-Difco) | 0.25 | g |
| Wolfe's mineral solution (see Medium No. 265) | 1.0 | ml |
| Modified Hutner's basal salts (see Medium No. 900) | 20.0 | ml |
| Artificial seawater | 250.0 | ml |
| Distilled water | 729.0 | ml |
Adjust pH to 7.0. After autoclaving, add the following filter-sterilized stock solutions to the medium (per liter):
| Glucose | 0.25 | g |
| N-Acetyl-D-glucosamine | 1.0 | g |
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
| Distilled water | 9.0 | ml |
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